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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
21/01/2015 |
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Data da última atualização: |
18/02/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOLINELLI, L. P.; CARVALHO, A. C.; CASAES, R. S.; LOPES, C. S. C.; DELIZA, R.; PASCHOALIN, V. M. F.; SILVA, J. T. |
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Afiliação: |
L. P. GOLINELLI, UFRJ; A. C. CARVALHO, UFRJ; R. S. CASAES, UFRJ; C. S. C. LOPES, UFRJ; ROSIRES DELIZA, CTAA; V. M. F. PASCHOALIN, UFRJ; J. T. SILVA, UFRJ. |
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Título: |
Sensory analysis and species-specific PCR detect bovine milk adulteration of frescal (fresh) goat cheese. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 97, n. 11, p. 6693-6699, Nov., 2014. |
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DOI: |
10.3168/jds.2014-7990 |
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Idioma: |
Inglês |
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Conteúdo: |
The Brazilian market for dairy products made from goat milk is increasing despite the seasonality of production and naturally small milk production per animal, factors that result in high-priced products and encourage fraud. In Brazil, no official analytical method exists for detecting adulteration of goat dairy products with cow milk. The aim of this study was to design a strategy to investigate the adulteration of frescal (fresh) goat cheeses available in the Rio de Janeiro retail market, combining analysis of cheese composition and the perception of adulteration by consumers. Commercial goat cheeses were tested by using a duplex PCR assay previously designed to authenticate cheeses, by targeting the mitochondrial 12S ribosomal RNA genes of both species simultaneously. The PCR test was able to detect 0.5% (vol/vol) cow milk added during goat cheese formulation. The analysis of 20 locally produced goat cheeses (20 lots of 4 brands) showed that all were adulterated with cow milk, even though the labels did not indicate the addition of cow milk. To estimate the ability of consumers to perceive the fraudulent addition of cow milk, a triangle test was performed, in which cheeses formulated with several different proportions of goat and cow milk were offered to 102 regular consumers of cheese. Detection threshold analysis indicated that almost half of the consumers were able to perceive adulteration at 10% (vol/vol) cow milk. Effective actions must be implemented to regulate the market for goat dairy products in Brazil, considering the rights and choices of consumers with respect to their particular requirements for diet and health, preference, and cost. MenosThe Brazilian market for dairy products made from goat milk is increasing despite the seasonality of production and naturally small milk production per animal, factors that result in high-priced products and encourage fraud. In Brazil, no official analytical method exists for detecting adulteration of goat dairy products with cow milk. The aim of this study was to design a strategy to investigate the adulteration of frescal (fresh) goat cheeses available in the Rio de Janeiro retail market, combining analysis of cheese composition and the perception of adulteration by consumers. Commercial goat cheeses were tested by using a duplex PCR assay previously designed to authenticate cheeses, by targeting the mitochondrial 12S ribosomal RNA genes of both species simultaneously. The PCR test was able to detect 0.5% (vol/vol) cow milk added during goat cheese formulation. The analysis of 20 locally produced goat cheeses (20 lots of 4 brands) showed that all were adulterated with cow milk, even though the labels did not indicate the addition of cow milk. To estimate the ability of consumers to perceive the fraudulent addition of cow milk, a triangle test was performed, in which cheeses formulated with several different proportions of goat and cow milk were offered to 102 regular consumers of cheese. Detection threshold analysis indicated that almost half of the consumers were able to perceive adulteration at 10% (vol/vol) cow milk. Effective actions must be implemented to regulate the... Mostrar Tudo |
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Palavras-Chave: |
Adulteração de leite de frescal; Análise Sensorial; Bovine and caprine species-specific PCR; Consumer detection threshold; Espécies específicas de PCR; Frescal (fresh) goat cheese; Queijo de cabra. |
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Thesaurus Nal: |
mitochondrial DNA. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02647naa a2200301 a 4500
001 2006285
005 2016-02-18
008 2014 bl uuuu u00u1 u #d
024 7 $a10.3168/jds.2014-7990$2DOI
100 1 $aGOLINELLI, L. P.
245 $aSensory analysis and species-specific PCR detect bovine milk adulteration of frescal (fresh) goat cheese.
260 $c2014
520 $aThe Brazilian market for dairy products made from goat milk is increasing despite the seasonality of production and naturally small milk production per animal, factors that result in high-priced products and encourage fraud. In Brazil, no official analytical method exists for detecting adulteration of goat dairy products with cow milk. The aim of this study was to design a strategy to investigate the adulteration of frescal (fresh) goat cheeses available in the Rio de Janeiro retail market, combining analysis of cheese composition and the perception of adulteration by consumers. Commercial goat cheeses were tested by using a duplex PCR assay previously designed to authenticate cheeses, by targeting the mitochondrial 12S ribosomal RNA genes of both species simultaneously. The PCR test was able to detect 0.5% (vol/vol) cow milk added during goat cheese formulation. The analysis of 20 locally produced goat cheeses (20 lots of 4 brands) showed that all were adulterated with cow milk, even though the labels did not indicate the addition of cow milk. To estimate the ability of consumers to perceive the fraudulent addition of cow milk, a triangle test was performed, in which cheeses formulated with several different proportions of goat and cow milk were offered to 102 regular consumers of cheese. Detection threshold analysis indicated that almost half of the consumers were able to perceive adulteration at 10% (vol/vol) cow milk. Effective actions must be implemented to regulate the market for goat dairy products in Brazil, considering the rights and choices of consumers with respect to their particular requirements for diet and health, preference, and cost.
650 $amitochondrial DNA
653 $aAdulteração de leite de frescal
653 $aAnálise Sensorial
653 $aBovine and caprine species-specific PCR
653 $aConsumer detection threshold
653 $aEspécies específicas de PCR
653 $aFrescal (fresh) goat cheese
653 $aQueijo de cabra
700 1 $aCARVALHO, A. C.
700 1 $aCASAES, R. S.
700 1 $aLOPES, C. S. C.
700 1 $aDELIZA, R.
700 1 $aPASCHOALIN, V. M. F.
700 1 $aSILVA, J. T.
773 $tJournal of Dairy Science$gv. 97, n. 11, p. 6693-6699, Nov., 2014.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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| Registros recuperados : 181 | |
| 6. |  | VASCONCELOS, E. A. R.; SILVA, T. S.; ROCHA, T. L.; GROSSI DE SÁ, M. F. 2D-E reference maps of celullar and secreted proteins from Bacillus thuringiensis (S811) during growth and sporulation. In: ANNUAL MEETING OF SBBQ, 37.; CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia, SP. Abstracts... Águas de Lindóia: SBBq, 2008. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | ZANOTTO, M. de; SAMPAIO, M. J. A.; JOHNSON, D. W.; ROCHA, T. L.; MARUNIAK, J. E. The Anticarsia gemmatalis nuclear polyhedrosis virus polyhedrin gene region: sequence analysis, gene product and structural comparisons. Journal of General Virology, Cambridge, v.73, n.5, p.1049-1056, 1992. p.1049-1056| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | ROCHA, T. L.; COSTA, P. H. A.; MAGALHÃES, J. C. C.; GROSSI DE SÁ, M. F. Análise proteômica de raízes de algodoeiro resistente e susceptível infectadas com Meloidogyne incognita. In: WORKSHOP INTERAÇÃO MOLECULAR PLANTA-PRAGAS, 2., 2007, Brasília, DF. II Workshop Interação Molecular Planta-Praga. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 113-118. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 229).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | SILVA, T.; VASCONCELOS, É. A. R.; ROCHA, T. L.; GROSSI-de-SÁ, M. F. Atividade quitinásica em extratos protéicos de Bacillus thuringiensis tóxicos para o bicudo do algodoeiro (Anthonomus grandis). In : ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 12., 2007, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 53.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | ASSIS, C. M.; OLIVEIRA, M. S.; SIHLER, W.; ROCHA, T. L.; CARNEIRO, M. Clonagem e expressão de fragmento de dna que codifica para a proteína rol a de Agrobacterium rhizogenes em sistema de expressão de levedura. In: WORKSHOP DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 4., 1999, Brasília. Anais: resumos dos trabalhos. Brasília, DF: EMBRAPA-CENARGEN, 1999. p. 46.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | SILVA, T. S.; VASCONCELOS, E. A. R.; ROCHA, T. L.; SA, M. F. G. de. Chitinolitic activity in proteic extracts of Bacillus thuringiensis toxic to boll weevil (Anthonomus grandis). In: ANNUAL MEETING OF SBBQ, 37.; CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia, SP. Abstracts... Águas de Lindóia: SBBq, 2008. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | LOURENÇO, I. T.; FRAGOSO, R. R.; ROCHA, T. L.; ELPÍDIO, W.; GROSSI-DE-SÁ, M. F. Construção de vetores para transformação genética de algodoeiro visando resistência à insetos-praga. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 74.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | LOURENÇO, I. T.; FRAGOSO, R. R.; ROCHA, T. L.; GROSSI DE SÁ, M. F. Construção de vetores para transformação genética de algodoeiro visando resistência a insetos-praga. In: WORKSHOP INTERAÇÃO MOLECULAR PLANTA-PRAGAS, 2., 2007, Brasília, DF. II Workshop Interação Molecular Planta-Praga. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 96-101. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 229).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | ROCHA, T. L.; PETERSON, G.; CRIMMINS, K.; BOYD, A.; SAWYER, L.; FOTHERGILL-GILMORE, L. A. Expression and secretion of recombinant ovine B-lactoglobulin in Saccharomyces cerevisiae and Kluyveromyces lactis. Biochemical Journal, London, v.313, n.3, p.927-932, 1996. p.927-932| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | FRAGOSO, R. da R.; NAKASU, E. Y. T.; ROCHA, T. L.; ROSA, A. J. de M. Genômica funcional. In: FALEIRO, F. G.; ANDRADE, S. R. M. de; REIS JUNIOR, F. B. dos. (Ed.). Biotecnologia: estado da arte e aplicações na agropecuária. Planaltina, DF: Embrapa Cerrados, 2011. 729 p. il. Color. p. 143-173| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | KOSOSKI, R. M.; REIS JÚNIOR, F. B.; FRANCO, O. L.; ROCHA, T. L.; MEHTA, A. Proteoma de Xanthomonas campestris pv. campestris na interação com genótipos resistente e suscetível de Brassica oleracea. Fitopatologia Brasileira, Brasília, DF, v. 32, S310, ago. 2007. Suplemento. Trabalho apresentado no 49. Congresso Brasileiro de Fitopatologia, 2007, Maringá, PR.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 181 | |
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